Canine Serum Alkaline Phosphatase Isoenzymes Detected by Polyacrylamide Gel Disk Electrophoresis.
نویسندگان
چکیده
منابع مشابه
Canine serum alkaline phosphatase isoenzymes detected by polyacrylamide gel disk electrophoresis.
Serum alkaline phosphatase (ALP) isoenzymes were studied in normal dogs using a commercially available polyacrylamide gel disk electrophoresis kit (PAG/disk kit). Serum samples taken from the dogs were incubated with neuraminidase, after which most showed ALP isoenzymes as two characteristic stained bands. To determine the origin of each band, ALP isoenzymes of serum and tissue extracts (liver,...
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A new isoenzyme of alkaline phosphatase (EC 3.1.3.1) has been reported to occur in sera from patients with lymphoproliferative diseases. This enzyme is character ized by an inability to hydrolyze cysteamine S-phosphate. We find that the 5,5 -dithobis(2-nitrobenzoic acid)-coupled assay method for cysteamine S-phosphate hydrolysis is not suitable for serum, and we were unable to confirm the exist...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2002
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.64.35